Types of homogenizer

Homogenization is a helpful technique in numerous applications, including particle size reduction, emulsification, tissue homogenization and dissociation, and cell disruption.

And with the wide variety of applications comes a list of different homogenizer technologies, each suitable for different uses. The main types of homogenizer technology are:. Some of the main points of difference in the various technologies that make them more suitable for one application or another including level of shear, sample capacity, throughput capacity, reproducibility, and scalability. Other considerations will include cost and ease-of-use.

types of homogenizer

Tissue homogenization. Cell disruption. Cellular organelle extraction. Microorganism extraction. Particle size reduction. Creating emulsions. Homogenization Under Cryogenic Conditions. A rotor-stator homogenizer comprises a metal shaft rotor which rotates rapidly within a stationary outer case stator. This creates a suction effect such that the sample is drawn into the space between the two components.

Here it is subject to very high-shear forces which help to homogenize the liquid or particles. A continuous process is formed as the sample is pushed through slots in the stator. The rapid motion of the fluid results in more sample entering the space to be subjected to shear.

These homogenizers are suitable for applications involving liquids and suspensions, including mixing or emulsifying. They can also be used for lysing cells and homogenizing soft tissue.

Ultrasonic homogenizers also known as sonicators also involve the use of a probe horn. The probe vibrates rapidly and transfers its ultrasonic energy to the surrounding sample. In addition, the sonication process results in cavitation, which involves the rapid formation and collapse of bubbles. This creates shockwaves which homogenize the surrounding sample. Ultrasonic homogenizers can achieve small particle sizes with relatively short processing times.

Using one of these homogenizers will result in a lot of noise being created so ear protection should be used. Common uses for ultrasonic homogenizers are particle size reduction, cell or tissue disruption, and DNA shearing.To save this word, you'll need to log in. Send us feedback. See More First Known Use of homogenizein the meaning defined at transitive sense 1a History and Etymology for homogenize see homogeneous Keep scrolling for more Learn More about homogenize Share homogenize Post the Definition of homogenize to Facebook Share the Definition of homogenize on Twitter Time Traveler for homogenize.

types of homogenizer

See more words from the same year Dictionary Entries near homogenize homogenetic homogenisation homogenization homogenize homogenized homogenous homogeny.

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Laboratory Homogenizers

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Login or Register. Save Word. Log In. Definition of homogenize. Keep scrolling for more. Other Words from homogenize homogenizer noun. Synonyms for homogenize Synonyms formalizenormalizeregularizestandardize Visit the Thesaurus for More.

Homogenizer

Examples of homogenize in a Sentence The new curriculum is an attempt to homogenize education throughout the county.The field of homogenizing encompasses a very broad area and is constantly evolving. The word homogenize means "to make or render homogeneous" while homogeneous means "having the same composition, structure or character throughout". Homogenizing is what is called an umbrella word - a word which covers a very large area.

When someone says that they are homogenizing, they may mean that they are actually doing one or more of the following, blending, mixing, disrupting, emulsifying, dispersing, stirring etc. Therefore, during this writing when the word homogenizing is used it may mean any one or more of the above-mentioned processes.

The current processes or methods of homogenizing can be broken down into three 3 major categories, mechanical, ultrasonic and pressure. Rotor-stator homogenizers also called colloid mills or Willems homogenizers generally outperform cutting blade-type blenders and are well suited for plant and animal tissue.

Combined with glass beads, the rotor-stator homogenizer has been successfully used to disrupt microorganisms. However, the homogenized sample is contaminated with minute glass and stainless steel particles and the abrasive wear to the rotor-stator homogenizer is unacceptably high. Cell disruption with the rotor-stator homogenizer involves hydraulic and mechanical shear as well as cavitation. Some people in the homogenizing field also claim that there is to a lesser extent high-energy sonic and ultrasonic pressure gradients involved.

I personally do not believe in the theory that high-energy sonic and ultrasonic pressure gradients are involved with mechanical homogenizers. The only thing that ultrasonic and mechanical rotor-stator homogenizing have in common is that both methods generate and use to some degree cavitation.

Cavitation is defined as the formation and collapse of low-pressure vapor cavities in a flowing liquid. Cavitation is generated as you move a solid object through a liquid at a high rate of speed. In mechanical homogenizing rotor-stator the blade rotor is being moved through the liquid at a high rate of speed generating cavitation. The rotor-stator homogenizer or generator type homogenizer was first developed to make dispersions and emulsions, and most biological tissues are quickly and thoroughly homogenized with this apparatus.

There the material is centrifugally thrown outward in a pump like fashion to exit through the slots or holes. Because the rotor blade turns at a very high rpm, the tissue is rapidly reduced in size by a combination of extreme turbulence, cavitation and scissor-like mechanical shearing occurring within the narrow gap between the rotor and the stator.

For the recovery of intracellular organelles or receptor site complexes, shorter times are used and the rotor speed is reduced. The variables to be optimized for maximum efficiency are as follows:. With mechanical, rotor-stator homogenizing the most important component is the homogenizer probe also known as the generator. The size of this precision crafter component can vary from the diameter of a pencil for 0.

Rotor s p eeds vary from 3, rpm for large units to 8, rpm for the smaller units. In principle, the rotor speed of the homogenizer should be doubled for each halving of the rotor diameter.

types of homogenizer

It is not the rpm's of the motor but the tip velocity of the rotor that is the important operating parameter. Other factors such as rotor-stator design, which there are many, materials used in construction, and ease of leaning are also important factors to consider in selecting a rotor stator homogenizer.

PRO Scientific's homogenizers have the following tip speeds for the various rotor stator generators:. The speed and efficiency of homogenization are greatly degraded by using too small a homogenizer, and the volume range over which a given homogenizer rotor stator size will function efficiently is only about 10 fold.

This must be balanced against the practical observation that concentrated samples, by colliding more frequently, are broken up more rapidly.Centri-Force supplies and refurbishes all makes of Homogenizer. We welcome enquiries from all market sectors that utilise homogenizer equipment and on request can provide professional guidance as to the type of machine most suited to your process. Our service team can provide on-site servicing and training as well as modifications to your equipment eliminating the need to remove the machine from site.

Homogenizers are widely used as a high pressure pump although they are mostly used for particle size reduction of materials. In the past we have used and tested materials from carrot juice to heavy mud used in oil rigs. Homogenizers are the most efficient laboratory tool for particle and droplet size reduction. They reduce materials into uniform-sized molecules through high-speed, high pressure maceration or cutting. Although the properties of the resulting material depend on the homogenizer and the original material, homogenizers generally are capable of reducing particles to sizes of 0.

In general, higher pressure during processing results in smaller sized particles. Many homogenizers can also be used for shredding, wetting, dissolving, emulsification, extraction, precipitation, cell rupture, and similar processes. Although homogenizers can be used with many different types of materials, they work best with materials that have high moisture, fat, or fibre content.

Some of their most common uses include industrial processing, pharmaceutical and cosmetic preparations, food service, and bioresearch and laboratory testing. There are a number of different types of homogenizer. They are capable of shearing large quantities of material in a long shaft that has angled knives at the bottom. The knives shred the material, which is then drawn up into a stator tip and ejected from small holes in the side of the stator to be sheared again and again.

This type of homogenizer tends to be quick and efficient and is capable of handling plant and animals tissues and other more solid materials for cell disruption and similar techniques. In general, materials are completely homogenized within 20 to 60 seconds. Foaming and aerosols can be a problem with certain types of materials. High pressure homogenizers are usually used with liquids and similar materials and this is the method most often used when homogenizing milk.

Sonic disruptors, or sonicators reduce break up particles with the disruptors, which generate intense ultrasonic waves ranging from about 18 to 50 kHz. Ultrasonic waves of this frequency are inaudible to the human ear, but capable of exerting pressures of more than atmospheres and temperatures of up to 5, degrees C.

The waves are amplified by a probe or horn into an intense beam that produces the cutting or shearing effect on particles through a process called cavitation. Under the right conditions, the pressure waves cause microscopic bubbles to form, which grow to a certain point and then collapse violently.

The implosion generates a shock wave that breaks cell membranes, reduces particle size, and even breaks covalent bonds.A premium membership for higher-level suppliers. Relevancy Transaction Level Response Rate. Supplier Types Trade Assurance. Supplier A premium membership for higher-level suppliers.

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Thank you for subscribing! Please check your inbox for a confirmation email to complete your signup. Laboratory Homogenizers. Designed for continuous operations, laboratory homogenizers are ideal for high pressure treatment of nano-particles, nano-dispersions, nano-emulsions and for cell disruption; they provide high performance up to a maximum pressure of bar which means that they are easily usable for different types of applications; biotechnology, pharmaceutical, cosmetics, chemical, dairy, food and beverage.

These devices offer the possibility of performing tests with small amounts of sample minimum volume of 0. View as: Grid List. This table top unit is dedicated to facilitate the testing and scaling of the NiSoX-Valve benefits in various applications while ensuring safe, smart and easy operating conditions.

Homogenizers Pilot Plant. Homogenizers Table-top. Table-top Homogenizers are compact machines ideal for the treatment of nanoparticles, nanodispersions and nanoemulsions. Mix, blend, homogenize, rinse and repeat.

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types of homogenizer

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The field of homogenizing

Cost occur over time while the machine is used. Keeping it running. GEA Remote Support. Video support designed to provide remote assistance in real-time. Adapting cutting-edge services to keep the global pharma industry on track. GEA, the globally active mechanical and plant engineering company, offers a suite of digital and virtual solutions to support the pharmaceutical industry as it embraces remote working and travel-free manufacturing.

BerlinGermany. Vaccine production technology plays a vital role in animal disease control. Stay in touch with GEA innovations and stories. Please hold on, we're processing your submission.A homogenizer is a piece of laboratory or industrial equipment used for the homogenization of various types of material, such as tissue, plant, food, soil, and many others.

Many different models have been developed using various physical technologies for disruption. The mortar and pestlealready used for thousands of years, is a standard tool even in modern laboratories. More modern solutions are based on blender type of instruments also known in the kitchenbead mills, ultrasonic treatment also sonicationrotor-stator mechanical, high pressure, and many other physical forces.

While there are many application overlaps between methods, each homogenization method has distinct advantages and disadvantages. Cell fractionation is done by homogenizer to release the organelles from cell. Whereas older technologies just focused on the disruption of the material, newer technologies also address quality or environmental aspects, such as cross-contamination, aerosols, risk of infection, or noise.

Homogenization is a very common sample preparation step prior to the analysis of nucleic acidsproteinscells, metabolismpathogensand many other targets. In the field of optics, a homogenizer is an optical device that makes the light beam from a laser or lamp source more uniform in its intensity across its cross-section to enable the light source to provide a more uniform illumination on a surface.

Use of a homogenizer in an illumination system is important in consumer applications such as light projectors for movies and industrial applications such as imaging equipment for microlithography for production of semiconductor microchips.

Such homogenizers are also called beam homogenizers or beam uniformizers. The main principle in their design approach is to divide the light beam cross-section-wise into multiple segments and then overlap these segments of different intensities into a recombined beam of improved uniformity. A variety of optical homogenization devices have been developed, including fly's-eye lens arrays, hollow and solid light tunnels, beam-folding wedged mirrors and split prisms.

From Wikipedia, the free encyclopedia. Mechanical homogenizer [ edit ] A homogenizer is a piece of laboratory or industrial equipment used for the homogenization of various types of material, such as tissue, plant, food, soil, and many others. Laboratory equipment.

Stands Clamps Holders. Dean—Stark Soxhlet extractor Kipp's. Boston round Pycnometer. Cold finger Liebig. Evaporating Petri Syracuse Watch glass. Beaker Bell jar Gas syringe Vial. Analytical chemistry. Personal protective equipment PPE. Lab coat Face shield Respirator Rubber apron Safety shower. Acid solvent cabinet Biosafety cabinet Fire blanket Fire extinguisher Fume hood. Instruments used in medical laboratories. Categories : Laboratory equipment. Namespaces Article Talk.

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